I Sce I-induced gene replacement and gene conversion in embryonic stem cells
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United States of America Patent
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Nov 3, 1998
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app pub date -
Aug 7, 1996
filing date -
Apr 18, 1996
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Abstract
This invention relates to a method of constructing a villin gene hybrid by inserting an I-Sce I restriction site next to or within a gene or cDNA encoding a villin protein. The insertion site of the I-Sce I restriction site is chosen as to provide a first downstream part and a second upstream part from the site, containing at least twelve nucleotides of the gene or cDNA encoding the villin protein. Furthermore, the insertion of the restriction permits a high frequency of homologous recombination events. The villin gene hybrid may be used to transfect eukaryotic cells, and particularly, embryonic stem cells.
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