Cloning and expression of the exo-polygalacturonase gene from aspergillus
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United States of America Patent
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Nov 3, 1998
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app pub date -
Jan 24, 1997
filing date -
Dec 24, 1992
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Abstract
The exo-polygalacturonase gene from Aspergillus is identified, and the enzyme encoded thereby is expressed. Specifically, the exo-polygalacturonase gene from Aspergillus tubingensis is is cloned and expressed, and DNA sequences from other Aspergillus strains that specifically hybridize therewith are identified. Novel expression vectors that comprise an exo-polygalacturonase encoding sequence, and host cells transformed therewith, are also provided. The invention further relates to the production of recombinant exo-polygalacturonase, and to the use of this protein.
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