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Abstract

Adsorptive sample preparation device and method effective for concentrating, desalting and/or purifying biomolecules. An adsorptive membrane is used to bind biomolecules, which biomolecules can then be eluted with a suitable desorption agent. In a preferred form, the adsorptive filter means is a particle-laden semipermeable membrane having at least one of its major surfaces, preferably both of its major surfaces, covered with a thin fabric. In the method of the invention for concentrating biomolecules or desalting or purify from a solution, the sample reservoir of the above-described adsorptive sample preparation device is filled with an analyte solution, placed in a fixed angle or swinging bucket centrifuge rotor, the solution is driven through the membrane, and analyte obtained as a result of adsorption on the membrane is recovered by washing and then eluting with a suitable desorption agent. A method of sealing the membrane in the device is also set forth.

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First Claim