Assay of blood or other biologic samples for target analytes

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United States of America Patent

PATENT NO 5834217
SERIAL NO

08763858

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ATTORNEY / AGENT: (SPONSORED)

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Abstract

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A patient's health may be diagnosed by centrifuging blood samples in a transparent tube, which tube contains one or more bodies or groups of bodies such as floats, inserts, liposomes, or plastic beads of different densities. Each density-defined body carries analyte-capture binding materials such as antigens or antibodies, which are specific to an epitope, or other specific high affinity binding site on a target analyte which target analyte may be in the blood or other sample being tested; and the level of which analyte is indicative of the patient's health. At least one labeled binding material which is also specific to an epitope, or other specific high affinity binding site on the target analyte is added to the sample so as to form labeled binding material/analyte/body complexes in the sample. Upon centrifugation, the complexes will settle out in different areas in the tube according to the respective density of the body or bodies; and the degree of label emission of the complex layers can enable qualitative and/or quantitative analyses of the sample to be made. Unbound labeled binding materials will be separated from the complexed layers by the washing action of ascending or descending components of the sample during the centrifugation step. Unbound labeled binding material will thus not interfere with the analysis.

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Patent Owner(s)

Patent OwnerAddress
QBC DIAGNOSTICS INC200 SHADYLANE DRIVE PHILIPSBURG PA 16866

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Dhanesar, Subhash Owings Mills, MD 2 158
Levine, Robert A Guilford, CT 145 3889
Lovell, Stephen J Baltimore, MD 8 361
Malick, Adrien P Granite, MD 9 271
Manion, Kristen L Benecia, CA 3 197
Ozinskas, Alvydas J Dayton, MD 4 305
Rodriguez, Rodolfo R Owings Mills, MD 29 684
Terstappen, Leon W M M Palo Alto, CA 41 2272
Wardlaw, Stephen C Old Saybrook, CT 181 4758

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