Quantification of nucleic acid
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United States of America Patent
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Nov 10, 1998
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N/A
app pub date -
Feb 7, 1995
filing date -
Aug 2, 1991
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Abstract
Disclosed is a method of quantifying a target nucleic acid in a test sample by adding to the test sample a known number of molecules of a corresponding nucleic acid comprising a well-defined mutant sequence. Said mutant sequence being discriminatory from the target nucleic acid. Subsequently a competitive amplification reaction of the nucleic acid is performed after which quantification of the amplified nucleic acid is performed by a differential detection.
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