Back-titration assay using two different markers
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United States of America Patent
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Nov 10, 1998
Grant Date -
N/A
app pub date -
Jun 14, 1996
filing date -
Dec 24, 1993
priority date (Note) -
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Abstract
A method for determining the concentration of an analyte 6 in a liquid sample is described. The method uses a binding agent 2 capable of binding the analyte 6 and first and second developing agents 8, 12, the first developing agent 8 capable of binding to unoccupied binding sites on the binding agent 2 and having a first marker 10, the second developing agent 12 capable of binding to the bound analyte or to the occupied binding sites and having a second marker 14 different from the first. The concentration of the analyte is determined by measuring the signals from the markers on first and second developing agents 8, 12. Thus, the method combines the competitive and non-competitive methods of the prior art and preferably has the advantage of extending the working range and/or sensitivity of the assay. A kit for performing the assay is also disclosed.
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