Flow cytometer calibration method
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United States of America Patent
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Nov 17, 1998
Grant Date -
N/A
app pub date -
Dec 27, 1995
filing date -
Dec 27, 1995
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Abstract
A method for calibrating a flow cytometer or fluorescence microscope and for quantitating binding proteins, such as antibodies. Populations of microbeads are provided which are in equilibrium with a saturating amount of fluorescently-labeled protein. The microbeads may have an incorporated fluorescent dye which has a strong signal in a selected channel of a flow cytometer, but no signal in other channels.
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