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Abstract

Apparatus for sampling a laser beam used in an analytical instrument, e.g., a clinical hematology or flow cytometer instrument, for measuring absorption by particles suspended in a stream moving through a flow cell. The laser beam is typically passed through a mask, e.g., a beam shaping aperture, to shape the beam. The shaped beam is then passed through a beam splitter, located downstream of the beam shaping aperture, so that one part of the shaped beam strikes the flow cell (e.g., is focused onto the moving stream of particles), and a second part is diverted to obtain a reference measurement of the beam about to strike the flow cell. An absorption measurement is obtained of the light passing through the flow cell, and a difference circuit is used to obtain a difference signal relating the differences between the measured absorption and the unabsorbed reference sample of the initial beam intensity. The laser source may be a laser diode and a spatial filter may be used to produce the laser beam to be passed through the beam shaping aperture.

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First Claim