Method of lyophilization of mammalian sperm cells
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United States of America Patent
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Dec 15, 1998
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N/A
app pub date -
Feb 15, 1991
filing date -
Feb 15, 1991
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Expired
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Abstract
A method is provided for the lyophilization of mammalian sperm cells which can be stored and reconstituted to provide morphologically intact cells. The method is advantageous in that the sperm cells may be stored at ambient temperatures for extended periods of time and recovered morphologically intact with DNA-containing heads and intact flagella. The lyophilization medium comprises a carbohydrate and a polymer selected from PVP, HES, dextran and poloxamers or mixtures thereof.
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