Protecting nucleic acids and methods of analysis
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United States of America Patent
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Jan 19, 1999
Grant Date -
N/A
app pub date -
Jun 6, 1996
filing date -
Dec 6, 1993
priority date (Note) -
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Abstract
A selected region of a nucleic acid is protected from attach by nuclease by completing to the nucleic acid a nucleic acid analogue of the PNA type. The surviving sequence may be detected in an assay, optionally after amplification. A PCR reaction may be sterilized and its product assayed by protecting a characterizing region of the product by PNA hybridization followed by nuclease degradation of unprotected nucleic acid.
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