Method of introducing exogenous compounds into cells by electroporation and apparatus for same
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United States of America Patent
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Feb 23, 1999
Grant Date -
N/A
app pub date -
Sep 23, 1996
filing date -
Sep 23, 1996
priority date (Note) -
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Abstract
An electroporation apparatus for introducing exogenous material into cells is described herein. The apparatus comprises first a base member (15) configured for holding a cell support, the cell support having a top surface portion, with the top surface portion configured for carrying adherent cells. The apparatus further comprises an electrode carrier (25) operably associated with the base member, the electrode carrier having a bottom surface portion, a first electrode (30) connected to the electrode carrier, and a second electrode (35) also connected to the electrode carrier. The electrode carrier has a channel (40) formed therein, with the channel positioned between the first electrode and the second electrode, so that exogenous material may be introduced through the channel and into contact with the cells. Methods for introducing exogenous compounds into a cell and for visually detecting the location of binding events within a cell are also disclosed.
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