Method for amplification of the response signal in a sandwich immunoassay
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United States of America Patent
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Mar 2, 1999
Grant Date -
N/A
app pub date -
Jun 10, 1996
filing date -
Jun 10, 1996
priority date (Note) -
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Abstract
Disclosed is an improvement to a sandwich type immunoassay in which there is immobilized to a solid support an antibody which is specific to an epitope of the analyte whose presence or concentration is being sought and a first labeled antibody which is specific to another epitope of the analyte. The improvement involves providing a second labeled antibody which is specific to the first labeled antibody to thereby form a chain of two or more labeled antibodies which results in amplification of the signal generated upon formation of the sandwich.
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