Method of producing a cell carrying an excess of mammalian centromeres and the cell line carrying an excess of mammalian centromeres
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United States of America Patent
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Apr 6, 1999
Issued Date -
N/A
app pub date -
Oct 21, 1996
filing date -
May 9, 1990
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Expired
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Abstract
DNA fragments and methods for obtaining them are disclosed which when put into mammalian cells together with a dominant marker gene are able to form functional centromeres. The sequences can be used to generate probes for these centromeres. Cell lines containing the functional centromeres are also provided. Methods are taught for isolating mammalian centromeric DNA as well as for producing cell lines carrying an excess of mammalian centromeres linked to a dominant selectable marker gene.
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