Apparatus for separation of nucleated blood cells from a blood sample
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United States of America Patent
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May 25, 1999
Issued Date -
N/A
app pub date -
Jul 23, 1997
filing date -
Oct 21, 1994
priority date (Note) -
Expired
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Abstract
A charge-flow separation apparatus (CFS) for enriching rare cell populations, particularly fetal cells, from a whole blood sample by separating the rare cell fractions from whole fractions according to the relative-charge density and/or the relative binding affinity for a leukocyte depletion solid phase matrix is described. The apparatus having an internal cooling system allows for dissipating heat generated by the electric field of the apparatus. The internal cooling system, consisting of a plurality of cooling tubes to circulate coolant material, prevents cellular degradation typically associated with the high heat generated by the electric field and permits the use of a higher voltage gradient to shorten separation times.
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