Testing for infestation of rapeseed and other cruciferae by the fungus Leptosphaeria maculans (blackleg infestation)
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United States of America Patent
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Jun 29, 1999
Grant Date -
N/A
app pub date -
Aug 29, 1995
filing date -
Mar 22, 1993
priority date (Note) -
Expired
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Abstract
A method of testing for infestation of tissue of rape or other Cruciferea with a virulent strain of Leptosphaeria maculans. The method involves isolating DNA of L. maculans from the tissue; subjecting the isolated DNA to amplification by the polymerase chain reaction (PCR) using sets of primers derived from LMR1 ?SEQ ID NO:11! (Genbank accession number M77515), a repetitive element of L. maculans specific to virulent strains of the fungus, to form a product containing amplified L. maculans DNA; preferably separating the amplified L. maculans DNA from the product; and detecting the presence of the amplified separated L. maculans DNA. The invention also relates to a method of deriving DNA of L. maculans from plant tissue for amplification by PCR, to the oligonucleotides forming the primers used in the PCR and to a diagnostic test kit which makes use of the method of testing.
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