Strand displacement amplification of RNA targets
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United States of America Patent
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Jun 29, 1999
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app pub date -
May 8, 1997
filing date -
Sep 21, 1995
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Abstract
Strand Displacement Amplification has been adapted to reverse transcription amplification of RNA targets. The method of the invention is referred to as reverse transcription SDA (rtSDA) and may be performed as a two-step process or as a one-step process in which cDNA copies of an RNA target sequence are generated and amplified concurrently. In the one-step process, rtSDA reaction conditions are such that a conventional reverse transcriptase and a DNA-dependent polymerase function together in a single reaction to produce cDNA and amplify it.
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