Cytoplasmic inhibition of gene expression

Number of patents in Portfolio can not be more than 2000

United States of America Patent

PATENT NO 5922602
SERIAL NO

08260546

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ATTORNEY / AGENT: (SPONSORED)

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Abstract

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The invention provides novel genetic constructions for the expression of inhibitory RNA in the cytoplasm of eukaryotic cells. The inhibitory RNA may be an anti-sense RNA or a co-suppressor RNA, and functions to reduce the expression of a gene of interest in the target cell. The genetic constructions of the invention are capable of replicating in the cytoplasm of a eukaryotic cell and comprise a promoter region in functional combination with an encoding polynucleotide. The genetic constructions may be designed so as to replicate in the cytoplasm of plant cells, yeast cells, and mammalian cells. When the eukaryotic cell of interest is a plant cell, the genetic construction is preferably derived from a plant RNA virus. Plant RNA virus derived genetic constructions may employ a plant virus subgenomic promoter, including subgenomic promoters from tobamoviruses in functional combination with the RNA encoding region. In a preferred embodiment of the invention, plant cells are induced to produce elevated levels of the carotenoid phytoene. The elevated levels of phytoene are achieved by inhibiting the expression of the enzyme phytoene desaturase using the vectors of the invention.

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First Claim

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Patent Owner(s)

Patent OwnerAddressTotal Patents
LARGE SCALE BIOLOGY CORPORATIONVACAVILLE, CA4

International Classification(s)

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
della-Cioppa, Guy Richard Vacaville, CA 4 49
Donson, Jonathan Davis, CA 16 95
Grill, Laurence K Vacaville, CA 28 345
Harvey, Damon Alan Vacaville, CA 2 27
Kumagai, Monto Hiroshi Davis, CA 3 48

Cited Art Landscape

Patent Info (Count) # Cites Year
 
CALGENE, INC. (2)
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KENTUCKY BIOPROCESSING, LLC (1)
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PIONEER HI-BRED INTERNATIONAL, INC. (1)
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BIONOVA HOLDING CORPORATION (A DELAWARE CORPORATION) (1)
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THE RESEARCH FOUNDATION OF STATE UNIVERSITY OF NEW YORK (2)
* 5190931 Regulation of gene expression by employing translational inhibition of MRNA utilizing interfering complementary MRNA 166 1989
* 5272065 Regulation of gene expression by employing translational inhibition of MRNA utilizing interfering complementary MRNA 56 1990
* Cited By Examiner

Patent Citation Ranking

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Patent Info (Count) # Cites Year
 
Other [Check patent profile for assignment information] (1)
* 2004/0214,318 Method for enhancing RNA or protein production using non-native 5' untranslated sequences in recombinant viral nucleic acids 1 2004
 
YALE UNIVERSITY (1)
7229829 Tobacco rattle virus vectors and related compositions and methods 14 2003
 
UNIVERSITY OF IOWA RESEARCH FOUNDATION (13)
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8329890 SiRNA-mediated gene silencing 8 2010
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* 2011/0212,520 RNA INTERFERENCE SUPPRESSION OF NEURODEGENERATIVE DISEASES AND METHODS OF USE THEREOF 11 2010
8779116 SiRNA-mediated gene silencing 6 2012
9260716 RNA interference suppression of neurodegenerative diseases and methods of use thereof 2 2013
9487779 siRNA-mediated gene silencing 0 2014
 
NORTH CAROLINA STATE UNIVERSITY (1)
6759571 Method of suppressing gene expression in plants 3 1999
 
THE GENERAL HOSPITAL CORPORATION (1)
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ALNYLAM PHARMACEUTICALS, INC. (4)
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8299042 Methods and compositions for silencing genes without inducing toxicity 3 2008
 
LARGE SCALE BIOLOGY CORPORATION (6)
* 6479291 Cytoplasmic inhibition of gene expression by viral RNA 8 1999
6303848 Method for conferring herbicide, pest, or disease resistance in plant hosts 5 1999
6800748 Cytoplasmic inhibition of gene expression and expression of a foreign protein in a monocot plant by a plant viral vector 0 2001
* 6720183 Cytoplasmic inhibition of gene expression 0 2002
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BIOSCOURCE TECHNOLOGIES, INC. (1)
6426185 Method of compiling a functional gene profile in a plant by transfecting a nucleic acid sequence of a donor plant into a different host plant in an anti-sense orientation 2 1999
 
KENTUCKY BIOPROCESSING, LLC (2)
7192740 Recombinant viral nucleic acids 0 2002
* 2004/0049,025 Recombinant viral nucleic acids 0 2002
 
The Samuel Roberts Noble Foundation (1)
* 2005/0039,228 Methods and compositions for analysis of plant gene function 8 2004
 
MEDICAL COLLEGE OF GEORGIA RESEARCH INSTITUTE, INC. (1)
* 2004/0147,475 Composition and method for in vivo and in vitro attenuation of gene expression using double stranded RNA 18 2004
 
GEORGIA HEALTH SCIENCES UNIVERSITY RESEARCH INSTITUTE, INC. (5)
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7888325 Composition and method for in vivo and in vitro attenuation of gene expression using double stranded RNA 3 2006
* 2009/0156,520 Composition and method for in vivo and in vitro attenuation of gene expression using double stranded RNA 0 2006
8148345 Composition and method for in vivo and in vitro attenuation of gene expression using double stranded RNA 0 2009
* 2009/0215,880 Composition and Method for IN VIVO and IN VITRO Attenuation of Gene Expression Using Double Stranded RNA 7 2009
 
IOWA RESEARCH FOUNDATION, INC. (1)
* 2004/0241,854 siRNA-mediated gene silencing 61 2003
 
iBio, Inc. and Fraunhofer USA, Inc. (1)
7683238 Production of pharmaceutically active proteins in sprouted seedlings 4 2002
 
NOVICI BIOTECH LLC (3)
6700040 Cytoplasmic gene inhibition or gene expression in transfected plants by a tobraviral vector 4 2001
7498480 Cytoplasmic gene inhibition or gene expression in transfected plants by a tobraviral vector 0 2003
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BAYER CROPSCIENCE N.V. (2)
* 7485774 Methods and means for delivering inhibitory RNA to plants and applications thereof 0 2002
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* 2004/0093,643 Production of pharmaceutically active proteins in sprouted seedlings 19 2002
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8148608 Systems and methods for clonal expression in plants 0 2005
* 2006/0085,871 Systems and methods for clonal expression in plants 8 2005
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* 2006/0277,634 Production of foreign nucleic acids and polypeptides in sprout systems 7 2006
8058511 System for expression of genes in plants 4 2008
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8597942 System for expression of genes in plants 3 2011
8951791 System for expression of genes in plants 1 2013
9551001 System for expression of genes in plants 0 2015
* Cited By Examiner