US Patent No: 5,922,602

Number of patents in Portfolio can not be more than 2000

Cytoplasmic inhibition of gene expression

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Abstract

The invention provides novel genetic constructions for the expression of inhibitory RNA in the cytoplasm of eukaryotic cells. The inhibitory RNA may be an anti-sense RNA or a co-suppressor RNA, and functions to reduce the expression of a gene of interest in the target cell. The genetic constructions of the invention are capable of replicating in the cytoplasm of a eukaryotic cell and comprise a promoter region in functional combination with an encoding polynucleotide. The genetic constructions may be designed so as to replicate in the cytoplasm of plant cells, yeast cells, and mammalian cells. When the eukaryotic cell of interest is a plant cell, the genetic construction is preferably derived from a plant RNA virus. Plant RNA virus derived genetic constructions may employ a plant virus subgenomic promoter, including subgenomic promoters from tobamoviruses in functional combination with the RNA encoding region. In a preferred embodiment of the invention, plant cells are induced to produce elevated levels of the carotenoid phytoene. The elevated levels of phytoene are achieved by inhibiting the expression of the enzyme phytoene desaturase using the vectors of the invention.

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First Claim

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Patent Owner(s)

Patent OwnerAddressTotal Patents
LARGE SCALE BIOLOGY CORPORATIONVACAVILLE, CA51

International Classification(s)

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  • [Patents Count]

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
della-Cioppa, Guy Richard 814 Derry Cir., Vacaville, CA 95688 4 32
Donson, Jonathan Oak Park, CA 16 53
Grill, Laurence K Vacaville, CA 28 232
Harvey, Damon Alan Vacaville, CA 1 19
Kumagai, Monto Hiroshi 1330 Brown Dr., Davis, CA 95616 2 32

Cited Art

Patent Info (Count) # Cites Year
 
CALGENE, INC. (2)
5,107,065 Anti-sense regulation of gene expression in plant cells 207 1988
5,453,566 Antisense regulation of gene expression in plant/cells 59 1991
 
THE RESEARCH FOUNDATION OF STATE UNIVERSITY OF NEW YORK (2)
5,190,931 Regulation of gene expression by employing translational inhibition of MRNA utilizing interfering complementary MRNA 136 1989
5,272,065 Regulation of gene expression by employing translational inhibition of MRNA utilizing interfering complementary MRNA 47 1990
 
BIONOVA HOLDING CORPORATION (A DELAWARE CORPORATION) (1)
5,231,020 Genetic engineering of novel plant phenotypes 158 1990
 
KENTUCKY BIOPROCESSING, LLC (1)
5,316,931 Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes 71 1992
 
PIONEER HI-BRED INTERNATIONAL, INC. (1)
5,316,930 Virus resistant plants having antisense RNA 11 1989

Patent Citation Ranking

Forward Cites

Patent Info (Count) # Cites Year
 
LARGE SCALE BIOLOGY CORPORATION (4)
6,479,291 Cytoplasmic inhibition of gene expression by viral RNA 3 1999
6,303,848 Method for conferring herbicide, pest, or disease resistance in plant hosts 5 1999
6,800,748 Cytoplasmic inhibition of gene expression and expression of a foreign protein in a monocot plant by a plant viral vector 0 2001
6,720,183 Cytoplasmic inhibition of gene expression 0 2002
 
FRAUNHOFER USA, INC. (2)
8,148,608 Systems and methods for clonal expression in plants 0 2005
8,058,511 System for expression of genes in plants 0 2008
 
GEORGIA HEALTH SCIENCES UNIVERSITY RESEARCH INSTITUTE, INC. (2)
7,888,325 Composition and method for in vivo and in vitro attenuation of gene expression using double stranded RNA 0 2006
8,148,345 Composition and method for in vivo and in vitro attenuation of gene expression using double stranded RNA 0 2009
 
NOVICI BIOTECH LLC (2)
6,700,040 Cytoplasmic gene inhibition or gene expression in transfected plants by a tobraviral vector 1 2001
7,498,480 Cytoplasmic gene inhibition or gene expression in transfected plants by a tobraviral vector 0 2003
 
ALNYLAM PHARMACEUTICALS, INC. (1)
8,299,042 Methods and compositions for silencing genes without inducing toxicity 0 2008
 
BAYER CROPSCIENCE N.V. (1)
7,485,774 Methods and means for delivering inhibitory RNA to plants and applications thereof 0 2002
 
BIOSCOURCE TECHNOLOGIES, INC. (1)
6,426,185 Method of compiling a functional gene profile in a plant by transfecting a nucleic acid sequence of a donor plant into a different host plant in an anti-sense orientation 2 1999
 
IBIO, INC. (1)
7,692,063 Production of foreign nucleic acids and polypeptides in sprout systems 1 2006
 
IBIO, INC. AND FRAUNHOFER USA, INC. (1)
7,683,238 Production of pharmaceutically active proteins in sprouted seedlings 1 2002
 
KENTUCKY BIOPROCESSING, LLC (1)
7,192,740 Recombinant viral nucleic acids 0 2002
 
NORTH CAROLINA STATE UNIVERSITY (1)
6,759,571 Method of suppressing gene expression in plants 2 1999
 
UNIVERSITY OF IOWA RESEARCH FOUNDATION (1)
8,329,890 SiRNA-mediated gene silencing 0 2010
 
YALE UNIVERSITY (1)
7,229,829 Tobacco rattle virus vectors and related compositions and methods 1 2003