Importance

Loading Importance Indicators...

Abstract

A container for growing cells in a culture medium is made by sealing two sheets of gas-permeable elastomeric material such as made of silicone together to enclose a culture chamber. A portion of one of the sheets is in the shape of a dome having a wall extending transverse to a major plane of that sheet. The thickness of the wall is greater than the thickness of the rest of the sheet. A permeable and porous body, preferably coated with a thin film of metal, may be in the culture chamber to provide a substrate for three-dimensional growth. A porous body can be implanted in an organ of an animal to allow dividing cells to grow into the porous body. The porous body is removed from the animal and placed in contact with other porous bodies in the culture chamber where cells grow from the porous body containing cells into the other porous bodies. Thereafter, the chamber can be opened and each body with cells combined with additional porous bodies so that additional cells can be grown to provide an almost unlimited source of cells from having started with the organ of only one animal.

Loading the Abstract Image...

First Claim