Process of producing B-lactam antibiotics applying microorganisms with increased ligase activity
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United States of America Patent
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Aug 24, 1999
Grant Date -
N/A
app pub date -
Jun 19, 1997
filing date -
Sep 28, 1994
priority date (Note) -
Expired
status (Latency Note)
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Abstract
The present invention relates to the biosynthesis of .beta.-lactam antibiotics. More specifically, the invention relates to processes of producing .beta.-lactam antibiotics in vivo and in vitro. Also contemplated is a novel enzyme capable of catalyzing certain steps involved in .beta.-lactam biosynthesis. Further, the invention relates to a DNA construct encoding the novel enzyme, a recombinant vector or transformation vehicle comprising the DNA construct, and finally a cell comprising the DNA construct or recombinant vector.
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