Fluorescent polymeric sensor for the detection of urea
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United States of America Patent
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Aug 31, 1999
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N/A
app pub date -
Aug 5, 1997
filing date -
Aug 5, 1997
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Expired
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Abstract
The present invention provides a urea sensor material having a first layer of a protonated pH sensitive fluorophore immobilized in a hydrophobic polymer, wherein the fluorophore can react quantitatively with ammonia and the transducing moiety of the fluorophore is neutrally charged when deprotonated; a second layer of urease and a polymer; and a third layer of a polymer. The present invention also provides a method for detecting urea using the urea sensor material and optical sensing devices that incorporate the urea sensor material.
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