Methods of in situ hybridization
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United States of America Patent
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Sep 7, 1999
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N/A
app pub date -
Jun 13, 1997
filing date -
Jun 13, 1997
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Expired
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Abstract
A method and single solution denaturation and hybridization solution useful with synthetic oligonucleotide probes for hybridizing DNA or RNA sequences by in situ hybridization. The inventive protocol employs synthetic oligonucleotide probes and a glycerol-based hybridization solution which is particularly useful in fluorescence labeling of chromosomes X, Y, 13, 18 and 21 in human lymphocytes, amniocytes and metaphase chromosomes, with the label being sufficiently stable to permit archival storage of the labeled sample for later hybridization or analysis.
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