Production of enzymatically active recombinant carboxypeptidase B
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United States of America Patent
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Sep 7, 1999
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N/A
app pub date -
Jan 13, 1997
filing date -
Jan 25, 1995
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Abstract
The subject invention provides a method of producing enzymatically active CPB which comprises treating a recombinant cell containing DNA encoding ProCPB, so that the DNA directs expression of the ProCPB, recovering from the cell the ProCPB so expressed, treating the recovered ProCPB under conditions permitting folding of the ProCPB, subjecting the folded ProCPB to enzymatic cleavage to produce enzymatically active CPB and purifying the enzymatically active CPB.
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