Device and method for DNA amplification and assay
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United States of America Patent
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Sep 7, 1999
Issued Date -
N/A
app pub date -
Jun 18, 1997
filing date -
Sep 12, 1995
priority date (Note) -
Expired
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Abstract
A DNA amplification and homogeneous DNA probe assay device is provided which includes a multiplicity of discrete sample cells in a flat 'card' format, with each sample cell containing the reagents necessary for both DNA amplification and homogeneous DNA probe assay. The device is particularly suitable for fluorescence polarization DNA probe assays, aid is preferably provided with and integal polarizer to avoid the need for polarizing elements in the related measuring apparatus. The size and geometry of the sample cells allows for a 'hot start' of the DNA amplification reaction and thereby avoids mis-priming of the amplification reaction.
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