Method for gene transfer into cells activated from a quiescent state
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United States of America Patent
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Sep 28, 1999
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N/A
app pub date -
Jul 10, 1997
filing date -
Dec 22, 1994
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Abstract
A method is disclosed for gene transfer into a culture of primitive stem cells which comprises a prestimulation step of adding a blocking agent to block at least one inhibitor of a cell cycle of said primitive stem cells. The prestimulation is time-limited for a period of less than approximately 36 hours so that said culture of primitive stem cells retains hematopoietic potential.
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