Method for introducing nucleic acids into higher eukaryotic cells
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United States of America Patent
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Oct 12, 1999
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N/A
app pub date -
Mar 6, 1997
filing date -
May 31, 1994
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Abstract
Process and medium for the transfection of higher eukaryotic cells with DNA/polycation complexes, wherein a medium is used which contains ethyleneglycol and/or glycerol. The medium may also contain a substance which prevents the acidification of the endosomes, and/or a lower alcohol such as ethanol. The process is particularly suitable for the transfection of primary cells such as fibroblasts. Stably transformed cells can be obtained thereby, e.g. tumour cells for use as tumour vaccines.
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