US Patent No: 5,965,794

Number of patents in Portfolio can not be more than 2000

Viral amplification of recombinant messenger RNA in transgenic plants

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Abstract

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A novel method of over expressing genes in plants is provided. This method is based on the RNA amplification properties of plus strand RNA viruses of plants. A chimeric multicistronic gene is constructed containing a plant promoter, viral replication origins, a viral movement protein gene, and one or more foreign genes under control of viral subgenomic promoters. Plants containing one or more of these recombinant RNA transcripts are inoculated with helper virus. In the presence of helper virus recombinant transcripts are replicated producing high levels of foreign gene RNA. Sequences are provided for the high level expression of the enzyme chloramphenicol acetyltransferase in tobacco plants by replicon RNA amplification with helper viruses and movement protein genes derived from the tobamovirus group.

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Patent Owner(s)

Patent OwnerAddressTotal Patents
BIOSOURCE TECHNOLOGIES, INC.VACAVILLE, CA24
KENTUCKY BIOPROCESSING, LLCVACAVILLE, CA26

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Turpen, Thomas H Vacaville, CA 63 375

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Patent Info (Count) # Cites Year
 
BOARD OF TRUSTEES OF MICHIGAN STATE UNIVERSITY (3)
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IBIO, INC. (3)
7,692,063 Production of foreign nucleic acids and polypeptides in sprout systems 2 2006
8,058,511 System for expression of genes in plants 1 2008
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HUAZHONG AGRICULTURAL UNIVERSITY (2)
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KENTUCKY BIOPROCESSING, LLC (2)
6,462,255 Viral amplification of recombinant messenger RNA in transgenic plants 1 1999
6,852,846 Viral amplification of recombinant messenger RNA in transgenic plants 0 2001
 
ENERGY, UNITED STATES DEPARTMENT OF (1)
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FRAUNHOFER USA, INC. (1)
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iBio, Inc. and Fraunhofer USA, Inc. (1)
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THE REGENTS OF THE UNIVERSITY OF MICHIGAN (1)
7,375,086 Modulators of Nod2 signaling 1 2006
 
WASHINGTON STATE UNIVERSITY (1)
6,878,861 Acyl coenzyme A thioesterases 0 2001

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