Viral amplification of recombinant messenger RNA in transgenic plants

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United States of America Patent

PATENT NO 5965794
SERIAL NO

08336724

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Abstract

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A novel method of over expressing genes in plants is provided. This method is based on the RNA amplification properties of plus strand RNA viruses of plants. A chimeric multicistronic gene is constructed containing a plant promoter, viral replication origins, a viral movement protein gene, and one or more foreign genes under control of viral subgenomic promoters. Plants containing one or more of these recombinant RNA transcripts are inoculated with helper virus. In the presence of helper virus recombinant transcripts are replicated producing high levels of foreign gene RNA. Sequences are provided for the high level expression of the enzyme chloramphenicol acetyltransferase in tobacco plants by replicon RNA amplification with helper viruses and movement protein genes derived from the tobamovirus group.

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Patent Owner(s)

Patent OwnerAddressTotal Patents
BIOSOURCE TECHNOLOGIES, INC.VACAVILLE, CA5
KENTUCKY BIOPROCESSING, LLCVACAVILLE, CA2

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Turpen, Thomas H Vacaville, CA 53 528

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Patent Info (Count) # Cites Year
 
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The United States of America as represented by the United States Department of Energy (1)
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Huazhong Agricultural University (2)
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IBIO, INC. (12)
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* 2005/0114,920 Expression of foreign sequences in plants using transactivation system 16 2004
8148608 Systems and methods for clonal expression in plants 0 2005
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7692063 Production of foreign nucleic acids and polypeptides in sprout systems 5 2006
* 2006/0277,634 Production of foreign nucleic acids and polypeptides in sprout systems 8 2006
8058511 System for expression of genes in plants 5 2008
* 2011/0070,609 Production of Foreign Nucleic Acids and Polypeptides in Sprout Systems 4 2010
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* Cited By Examiner