US Patent No: 5,965,794

Number of patents in Portfolio can not be more than 2000

Viral amplification of recombinant messenger RNA in transgenic plants

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Abstract

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A novel method of over expressing genes in plants is provided. This method is based on the RNA amplification properties of plus strand RNA viruses of plants. A chimeric multicistronic gene is constructed containing a plant promoter, viral replication origins, a viral movement protein gene, and one or more foreign genes under control of viral subgenomic promoters. Plants containing one or more of these recombinant RNA transcripts are inoculated with helper virus. In the presence of helper virus recombinant transcripts are replicated producing high levels of foreign gene RNA. Sequences are provided for the high level expression of the enzyme chloramphenicol acetyltransferase in tobacco plants by replicon RNA amplification with helper viruses and movement protein genes derived from the tobamovirus group.

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Patent Owner(s)

Patent OwnerAddressTotal Patents
BIOSOURCE TECHNOLOGIES, INC.VACAVILLE, CA5
KENTUCKY BIOPROCESSING, LLCVACAVILLE, CA2

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Turpen, Thomas H Vacaville, CA 53 463

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Patent Info (Count) # Cites Year
 
THE REGENTS OF THE UNIVERSITY OF MICHIGAN (2)
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BROOKHAVEN SCIENCE ASSOCIATES, LLC (1)
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BOARD OF TRUSTEES OF MICHIGAN STATE UNIVERSITY (7)
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* 2009/0061,492 Method for producing biodiesel 13 2007
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CONTROLS CORPORATION OF AMERICA, INC. (1)
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ENERGY, UNITED STATES DEPARTMENT OF (1)
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iBio, Inc. and Fraunhofer USA, Inc. (1)
7,683,238 Production of pharmaceutically active proteins in sprouted seedlings 3 2002
 
KENTUCKY BIOPROCESSING, LLC (3)
* 6,462,255 Viral amplification of recombinant messenger RNA in transgenic plants 1 1999
6,852,846 Viral amplification of recombinant messenger RNA in transgenic plants 0 2001
* 2002/0104,123 Viral amplification of recombinant messenger RNA in transgenic plants 0 2001
 
WASHINGTON STATE UNIVERSITY (2)
6,878,861 Acyl coenzyme A thioesterases 0 2001
* 2003/0028,915 Acyl coenzyme a thioesterases 16 2001
 
HUAZHONG AGRICULTURAL UNIVERSITY (2)
7,834,244 Transcription factor gene OsNACx from rice and use thereof for improving plant tolerance to drought and salt 2 2005
8,378,173 Transcription factor gene OsNACx from rice and use thereof for improving plant tolerance to drought and salt 0 2010
 
iBio Inc. (10)
* 2004/0093,643 Production of pharmaceutically active proteins in sprouted seedlings 18 2002
* 2005/0114,920 Expression of foreign sequences in plants using transactivation system 14 2004
8,148,608 Systems and methods for clonal expression in plants 0 2005
* 2006/0085,871 Systems and methods for clonal expression in plants 7 2005
7,692,063 Production of foreign nucleic acids and polypeptides in sprout systems 3 2006
* 2006/0277,634 Production of foreign nucleic acids and polypeptides in sprout systems 6 2006
8,058,511 System for expression of genes in plants 3 2008
* 2011/0070,609 Production of Foreign Nucleic Acids and Polypeptides in Sprout Systems 2 2010
8,597,942 System for expression of genes in plants 2 2011
8,951,791 System for expression of genes in plants 0 2013
* Cited By Examiner