Promoter of M. paratuberculosis and its use for the expression of immunogenic sequences
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United States of America Patent
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Oct 19, 1999
Grant Date -
N/A
app pub date -
Oct 6, 1994
filing date -
Oct 25, 1991
priority date (Note) -
Expired
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Importance
Abstract
The invention relates to a nucleotide sequence which is present at a position adjacent to the 5' end of the reverse sequence complementary to the open reading frame coding for a potential transposase contained in the insertion element IS900 in Mycobacterium paratuberculosis. The nucleotide sequence has promoter functions and contains important signals for the regulation of transcription and translation. The invention also relates to methods for cloning and expressing heterologous proteins using such regulatory sequences, to vectors and transformed host cells containing these sequences, and to immunogenic compositions prepared by expression of nucleotide sequences placed under control of these regulatory sequences.
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