Soybean transformation and regeneration methods
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United States of America Patent
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Oct 19, 1999
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Mar 28, 1997
filing date -
Mar 28, 1997
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Abstract
A method of transforming and regenerating soybean plants relies on selection of hypocotyl explants as the target material. Hypocotyl explants can be transformed either by microparticle bombardment with DNA-coated microparticles of inert metals, or by co-culturing with an Agrobacterium strain. The transformed explants can be successfully regenerated, using a protocol including culturing on a shoot induction medium, followed by transfer to a shoot elongation medium to form rooted plantlets, which are transplanted to soil.
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