Transfection and genetic manipulations in obligate intracellular parasites
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United States of America Patent
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Nov 2, 1999
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app pub date -
May 31, 1996
filing date -
Nov 4, 1993
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Abstract
A method of transient transfection of obligate intracellular parasites is described. This method is exploited to develop a system for stable transformation utilizing selectable genes. For example, introduction of the chloramphenicol acetyl transferase (CAT) gene fused to Toxoplasma flanking sequences followed by chloramphenicol selection results in parasites stably expressing CAT. DNA hybridization analysis indicated that the CAT gene had inserted via homologous recombination.
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