Methods for producing recombinant mammalian cells harboring a yeast artificial chromosome
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United States of America Patent
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Nov 9, 1999
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N/A
app pub date -
Jan 25, 1994
filing date -
Jan 7, 1993
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Abstract
The present invention provides methods and compositions for transferring large transgene polynucleotides and unlinked selectable marker polynucleotides into eukaryotic cells by a novel method designated co-lipofection. The methods and compositions of the invention are used to produce novel transgenic non-human animals harboring large transgenes, such as a transgene comprising a human APP gene or human immunoglobulin gene.
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