Calmodulin-based cell separation technique
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United States of America Patent
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Nov 16, 1999
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N/A
app pub date -
Nov 14, 1997
filing date -
Nov 14, 1997
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Expired
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Abstract
The invention provides a method for separating target cells from a plurality of cells which is based on a reversible high affinity interaction between two molecules. The method comprises: forming a target cell/cell binding reagent/first molecule/second molecule/solid support complex, wherein the cell binding reagent is specific for target cells present within a plurality of cells and wherein the first molecule reversibly binds to the second molecule; removing non-target cells of the plurality of cells not attached to the solid support; and reversing the first molecule binding to the second molecule, thereby releasing the target cells as separate cells from the plurality of cells.
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