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Abstract

This invention relates to novel oligonucleotide-polyamide conjugates preferably having a free 3' hydroxl moiety, and wherein the polyamide is coupled to the oglionucleotide through its carboxyl terminus. A nucleotide polymer conjugate of the formula (I): Nu--NUC--C.dbd.C--X.sup.1 --NH--X.sup.2 --X.sup.3 where X.sup.1 is an unsubstituted or substituted C.sub.1 -C.sub.10 alkylene group, in which one or more carbons may optionally be replaced by --NH--, --O-- or --S--; X.sup.2 is a bond, or an unsubstituted or substituted C.sub.1 -C.sub.20 alkylene group, in which one or more carbons may optionally be replaced by --NH--, --O-- or --S--; the optional substituents in X.sup.1 or X.sup.2 are selected from a variety of groups; X.sup.3 is an amino acid or a polyamide linked via its carboxy terminus; NUC is a nucleoside group of any one of formulas (a), (b), (c), (d) where.fwdarw.indicates the bond to the --C--C-- group in formula (I), and X.sup.4 is a sugar group of formula(e) where the 5' oxygen is linked to Nu, and X.sup.5 and X.sup.6 are each independently H or OR, where R is H, a protecting group, or a solid phase matrix, and Nu is an oligonucleotide. Methods of preparing these compounds, and various uses, for example, as PCR primers, or as a substrate for DNA or RNA polymerase, are also disclosed.

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First Claim