Method for inducing bone formation using an extract of human osteosarcoma cell line SAOS-2
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United States of America Patent
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Feb 1, 2000
Issued Date -
N/A
app pub date -
Feb 8, 1993
filing date -
Oct 9, 1987
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Expired
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Abstract
The invention relates to a method for inducing bone formation via administration of an extract of devitalized, freeze dried Saos-2 cells. The extract is prepared by contacting the freeze dried Saos-2 cells, which are devitalized in the act of freeze drying, with a weak denaturing agent of the type used to separate proteins from cells. The extract does have properties superior to comparable treatment using whole Saos-2 cells. The extract is then further treated by applying it to a removing separating gel, and then fractions from the gel which have a molecular weight of from about 10 kd to about 60 kd. Also described are the extract itself, and formulations containing it.
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