Situ hybridization slide processes
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United States of America Patent
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Feb 8, 2000
Issued Date -
N/A
app pub date -
Oct 9, 1996
filing date -
Apr 7, 1995
priority date (Note) -
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Abstract
A method of preparing flourescent in situ hybridization slides, comprising: providing a slide with a raised ring thereon; providing to the slide a DNA probe; providing to the slide cells and target DNA; providing to the slide a formamide-free mixture of 10%.+-.2% by weight dextran sulfate and 15%-25% glycerol and 0.9% by weight salt or a solution of 10%.+-.2% by weight dextran sulfate, 10-30% by volume formamide and 0.9% by weight salt; placing a coverglass on the ring; denaturing and hybridizing the slide; and performing a post-hybridization wash of the slide.
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