Detection of ligand Interaction with polymeric material
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United States of America Patent
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Mar 7, 2000
Grant Date -
N/A
app pub date -
Jun 20, 1997
filing date -
Jun 20, 1996
priority date (Note) -
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Abstract
A method and apparatus for measuring ligand binding to DNA or protein uses changes in the mechanical properties of strips of the DNA or protein to detect the formation of ligand complexes. Samples strips are prepared by pouring protein solution onto a glass surface, drying the solution, and causing the protein to be cross-linked; the cross-linked film is scraped off, cut into strips, and mounted in the stress/strain measuring apparatus, which includes force and strain transducers. The sample is held by arms and can be immersed into solutions. The arms are attached to transducers. The apparatus includes a sample holder. The sample films may include end reinforcements and may be pinned or glued to the arms.
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