Method for more efficient electroporation
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United States of America Patent
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Mar 21, 2000
Grant Date -
N/A
app pub date -
Feb 22, 1999
filing date -
Oct 9, 1998
priority date (Note) -
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Abstract
This invention provides improved electroporation methods for transferring nucleic acids of interest into host cells, wherein the host cells are (1) suspended in a substantially non-ionic solution comprising at least one sugar or sugar derivative, (2) mixed with the nucleic acids of interest, and (3) electrically treated. Also, this invention provides for kits used in the method for transferring nucleic acids into host cells.
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