Diagnostic kits and methods for making granulocyte cell counts
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United States of America Patent
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Apr 4, 2000
Issued Date -
N/A
app pub date -
Jun 10, 1992
filing date -
Jul 9, 1991
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Expired
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Abstract
Methods for detecting granulocytes and for providing an at least semiquantitative indication of granulocyte level and kits for implementing those methods. A blood sample is drawn, and components that might interfere with the assay are removed and/or neutralized. Granulocytes present in the sample are then disrupted to release intracellular myeloperoxidase, and the sample is contacted with a peroxide and a chromogenic donor dye. The myeloperoxidase enzyme catalyzes hydrogen peroxide-involved reactions which result in the dye changing color if, and to the extent that, granulocytes are present in the blood sample.
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