Reagent and method for isolation and detection of selected nucleic acid sequences
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United States of America Patent
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May 9, 2000
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N/A
app pub date -
Nov 13, 1997
filing date -
Nov 15, 1996
priority date (Note) -
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Abstract
The invention relates to compositions and methods for rapidly detecting or isolating a target nucleic acid sequence in a polynucleotide-containing sample. The sample is exposed to a rapid pairing reagent, which contains a rapid capture component, effective to rapidly and non-selectively bind polynucleotides, and a target specific probe, effective to selectively bind the target nucleic acid sequence. Selectively disrupting the binding between the capture component and polynucleotides leaves only target sequence bound to the rapid pairing reagent.
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