Target nucleic acid sequence amplification
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United States of America Patent
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May 16, 2000
Issued Date -
N/A
app pub date -
Oct 14, 1997
filing date -
Mar 18, 1996
priority date (Note) -
Expired
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Abstract
A method is provided for the rapid, substantially isostatic, segregation and amplification of the sequence information of a target nucleic acid sequence positioned within a single- or double-stranded polynucleotide. The method is based on the serial generation of double-stranded DNA engineered to contain terminal nicking sites, nicking of those sites, and extensions from those nicks, thereby displacing any existing polynucleotides. Further provided is a method for detecting polynucleotides using the method of the invention. A kit combining the components commonly used in practicing the method of the invention is also provided.
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