DNA sequencing by mass spectrometry via exonuclease degradation
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United States of America Patent
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Jun 13, 2000
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app pub date -
Nov 6, 1996
filing date -
Mar 19, 1993
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Abstract
The invention provides fast and highly accurate mass spectrometer based processes for directly sequencing a target nucleic acid (or fragments generated from the target nucleic acid), which by means of protection, specificity of enzymatic activity, or immobilization, are unilaterally degraded in a stepwise manner via exonuclease digestion and the nucleotides, derivatives or truncated sequences detected by mass spectrometry.
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