Process for transforming monocotyledonous plants
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United States of America Patent
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Jun 13, 2000
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N/A
app pub date -
May 28, 1998
filing date -
Nov 23, 1990
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Abstract
A process for the stable integration of a DNA, comprising a gene that is function in a cell of a cereal plant, wherein said DNA is integrated into the nuclear genome of said cereal plant, said process comprising the steps of: (a) providing a compact embryogenic callus of said cereal plant; (b) wounding said compact embryogenic callus or treating said compact embryogenic callus with a cell wall degrading enzyme for a period of time so as not to cause a complete disruption of tissues, and transferring said DNA into the nuclear genome of a cell in said compact embryogenic callus by means of Agrobacterium-mediated transformation to generate a transformed cell; and (c) regenerating a transformed cereal plant from said transformed cell.
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