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Abstract

Disclosed is a method of producing a compound which contains the Glc.beta.1-4GlcN structure involving reacting at least one donor substance Glc.beta.OR where R is an organic group, and at least one acceptor substance which is a glucopyranosamino derivative having the formula GlcNR'-R'', wherein NR' is an azido, 2-N-acetyl-, 2-N-phtalimido, or an organic group bound to the 2-N-group of glucosamine, wherein R'' is a glycosidically bound fluoro or is an O-, C-, N- or S-glycosidically bound aliphatic or aromatic compound, with the optional proviso that if NR' is NHAc then R'' is not OH and if NR' is not NHAc then R'' may be OH, in the presence of Bullera singularis or an E.C. group 3.2 glycosidase of essentially the same structure as an E.C. group 3.2 glucosidase obtained from Bullera singularis to form the Glc.beta.1-4GlcN derivative; and optionally isolating the compound which contains the Glc.beta.1-4GlcN structure.

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First Claim