Recombinant expression of proteins from secretory cell lines
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United States of America Patent
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Jul 11, 2000
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N/A
app pub date -
Jan 19, 1996
filing date -
Jan 19, 1996
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Abstract
The present invention a provides methods for production of heterologous polypeptides using a variety recombinantly engineered secretory cell lines. The common feature of these cell lines is the absence of expression of at least one endogenous polypeptide. The host cell machinery normally used to produce the endogenous polypeptide is then usurped for the purpose of making the heterologous polypeptide. Also described are methods engineering cells for high level expression, methods of large scale protein production, and methods for treatment of disease in vivo using viral delivery systems and recombinant cell lines.
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