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Abstract

Useful ingredients are extracted from a mycelium-containing culture medium by the steps of inoculating basidiomycetes in a solid culture medium containing bagasse as a substrate and proliferating mycelium, and then squeezing the solid culture medium containing proliferated mycelium to obtain a squeeze liquid (i), separately, adding water and beta 1,3, glucanase and at least one enzyme selected from the group consisting of chitinase, cellulase and protease to a mycelium-containing solid residue having been separated from the squeeze liquid (i), stirring the resulting mixture with maintaining the mixture at 30 to 60.degree.0 C. to lyse mycelial cell walls, and heating the resulting cell wall lysis product-containing liquid (ii) up to a temperature of 95.degree. C. to inactivate the enzyme and perform sterilization. The cell wall lysis product-containing liquid (ii) contains .beta.-glucan in a high concentration and shows excellent antitumor effects. The obtained squeeze liquid (i) contains cytokinin-like substances in high concentrations together with metabolites of the mycelium, and this liquid is clear; therefore, application of this liquid to tonic drinks, plant hormone drugs and cosmetic materials can be expected.

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First Claim