US Patent No: 6,090,784

Number of patents in Portfolio can not be more than 2000

RNA polymerase II peptides and methods of use

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ATTORNEY / AGENT: (SPONSORED)

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Abstract

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A macromolecular delivery method that utilizes a series of peptides with unique and versatile nuclear targeting properties has been developed, where the peptides are derived from the COOH terminal domain (CTD) of the largest subunit of RNA polymerase II and include heptapeptide units similar or identical to the following consensus sequence: Tyrosine--Serine--Proline--Threonine--Serine--Proline--Serine (YSPTSPS).sub.x (SEQ ID NO. 1). When expressed in vivo, the CTD peptides are phosphorylated and they accumulate in discrete compartments within the nucleus. The CTD peptides concentrate indicator molecules in discrete subnuclear compartments where pre-mRNA molecules are synthesized and spliced. The length and composition of the CTD peptides can be manipulated to obtain different intranuclear partitioning properties. The CTD peptides are functional in the nuclei of S. cerevisiae, S. pombe, nematodes, insects, plants, and all vertebrates. Since the CTD peptides accumulate precisely in discrete sites inhabited by RNA polymerase II and the spliceosomes, they should be useful in genetic therapy technologies.

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Patent Owner(s)

Patent OwnerAddressTotal Patents
YALE UNIVERSITYNEW HAVEN, CT673

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Warren, Stephen L Orange, CT 17 68

Cited Art Landscape

Patent Info (Count) # Cites Year
 
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UNIVERSITY OF GEORGIA RESEARCH FOUNDATION, INC. (1)
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Patent Citation Ranking

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FBP Corporation (1)
6,461,641 Tumor delivery vehicles 2 1999
 
MERCK PATENT GMBH (1)
7,262,283 Method for extracting proteins 2 2003