RNA polymerase II peptides and methods of use

Number of patents in Portfolio can not be more than 2000

United States of America Patent

PATENT NO 6090784
SERIAL NO

08566190

Stats

ATTORNEY / AGENT: (SPONSORED)

Importance

Loading Importance Indicators... loading....

Abstract

See full text

A macromolecular delivery method that utilizes a series of peptides with unique and versatile nuclear targeting properties has been developed, where the peptides are derived from the COOH terminal domain (CTD) of the largest subunit of RNA polymerase II and include heptapeptide units similar or identical to the following consensus sequence: Tyrosine--Serine--Proline--Threonine--Serine--Proline--Serine (YSPTSPS).sub.x (SEQ ID NO. 1). When expressed in vivo, the CTD peptides are phosphorylated and they accumulate in discrete compartments within the nucleus. The CTD peptides concentrate indicator molecules in discrete subnuclear compartments where pre-mRNA molecules are synthesized and spliced. The length and composition of the CTD peptides can be manipulated to obtain different intranuclear partitioning properties. The CTD peptides are functional in the nuclei of S. cerevisiae, S. pombe, nematodes, insects, plants, and all vertebrates. Since the CTD peptides accumulate precisely in discrete sites inhabited by RNA polymerase II and the spliceosomes, they should be useful in genetic therapy technologies.

Loading the Abstract Image... loading....

First Claim

See full text

Family

Loading Family data... loading....

Patent Owner(s)

Patent OwnerAddressTotal Patents
YALE UNIVERSITYNEW HAVEN, CT565

International Classification(s)

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Warren, Stephen L Orange, CT 15 180

Cited Art Landscape

Patent Info (Count) # Cites Year
 
DUKE UNIVERSITY (1)
* 5565327 Methods of diagnosing parasitic infections and of testing drug susceptibility of parasites 1 1994
 
UNIVERSITY OF GEORGIA RESEARCH FOUNDATION, INC. (1)
* 4629784 Synthesis of cyclopropane amino acids 30 1984
 
La Jolla Cancer Research Foundation (1)
* 4792525 Tetrapeptide 191 1985
 
The Salk Institute for Biological Studies (1)
* 4316891 Extended N-terminal somatostatin 59 1980
* Cited By Examiner

Patent Citation Ranking

Forward Cite Landscape

Patent Info (Count) # Cites Year
 
FBP Corporation (1)
* 6461641 Tumor delivery vehicles 2 1999
 
YEDA RESEARCH AND DEVELOPMENT CO. LTD. (3)
8748371 Nuclear targeting sequences 0 2008
* 2010/0099,627 NUCLEAR TARGETING SEQUENCES 1 2008
9315547 Nuclear targeting sequences 0 2014
 
MERCK PATENT GMBH (2)
* 7262283 Method for extracting proteins 4 2003
* 2006/0166,267 Method for extracting proteins 0 2003
* Cited By Examiner