Methods and reagents for typing HLA class I genes
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United States of America Patent
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Aug 15, 2000
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app pub date -
Oct 3, 1995
filing date -
Feb 14, 1995
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Abstract
A method for typing HLA class 1 genes. The method entails first contacting a sample DNA with first and second amplification primers, wherein the first and second primers are each at least partially located in an exonic region. Next, using the first and second primers, a target sequence is amplified by the PCR to form an amplicon of the target sequence. Finally, the amplicon is detected with a sequence-specific detection means, e.g., DNA sequencing. The invention also includes specific amplification primers, specific sequencing primers, and kits especially adapted for use with the above HLA typing method.
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