Biosensor device and method
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United States of America Patent
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-
Aug 22, 2000
Grant Date -
N/A
app pub date -
Apr 24, 1997
filing date -
Apr 25, 1996
priority date (Note) -
Expired
status (Latency Note)
Importance
Abstract
A biosensor apparatus for detecting a binding event between a ligand and receptor. The apparatus includes an electrode substrate coated with a high-dielectric hydrocarbon-chain monolayer, and having ligands attached to the exposed monolayer surface. Binding of a receptor to the monolayer-bound ligand, and the resultant perturbation of the monolayer structure, causes ion-mediated electron flow across the monolayer. In one embodiment, the monolayers have a coil-coil heterodimer embedded therein, one subunit of which is attached to the substrate, and the second of which carries the ligand at the monolayer surface.
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