Method for introducing pharmaceutical drugs and nucleic acids into skeletal muscle
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United States of America Patent
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Aug 29, 2000
Grant Date -
N/A
app pub date -
Apr 3, 1998
filing date -
Apr 3, 1997
priority date (Note) -
In Force
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Abstract
A method is disclosed for delivering molecules such as pharmaceutical drugs and nucleic acids into skeletal muscle in vivo. The pharmaceutical drug or nucleic acid is first injected into the muscle at one or multiple sites. Immediately, or shortly after, injection, electrodes are placed flanking the injection site and a specific amount of electrical current is passed through the muscle. The electrical current makes the muscle permeable, thus allowing the pharmaceutical drug or nucleic acid to enter the cell. The efficiency of transfer permits robust immune responses using DNA vaccines and produces sufficient secreted proteins for systemic biological activity to be observed.
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