Sequence modification of oligonucleotide primers to manipulate non-templated nucleotide addition
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United States of America Patent
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Aug 29, 2000
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N/A
app pub date -
Apr 14, 1998
filing date -
Oct 15, 1996
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Abstract
The present invention is directed to methods for resisting or promoting template independent nucleotide addition to the 3' terminus of a DNA duplex. The process comprises amplifying a target nucleic acid using primers which comprise a 5' terminal sequence which resists or promotes non-templated nucleotide addition to the 3' terminus of the complementary nucleic acid strand. The invention is also directed to a kit for cloning 3' nucleotidylated duplex DNA.
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