Nucleic acid sequencing and mapping
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United States of America Patent
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Sep 12, 2000
Grant Date -
N/A
app pub date -
Mar 6, 1997
filing date -
Mar 5, 1997
priority date (Note) -
Expired
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Abstract
An improvement over the standard Sanger Method for nucleic acid sequencing is described. The novel method does not require denaturation of double-stranded template; rather, sequencing can be carried out directly on the double-stranded template. Embodiments are described with and without oligonucleotide primers.
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